Gram-negative bacilli (GNB) are most frequently isolated bacteria from ascitic fluid aspirate of patients with Spontaneous bacterial peritonitis (SBP). Aetiology of SBP has been changing in recent years with increased involvement of multidrug resistant (MDR) bacteria as a result of production of various β-lactamases. The present study was done for detection of different β-lactamases and their co-existence by noveldisc placement method among GNB isolates from patients with SBP. Disc panel containing ceftazidime (CA), ceftazidime/tazobactam (CA/TZ), cefepime (CFP), ceftazidime/ tazobactam/ cloxacillin (CA/TZ/CLOX), imipenem (IMP) and Mercaptopropionic acid (MPA) was used. Pureextended spectrum β-lactamase (ESBL) production was found in 18 of 42 isolates (). Maximum ESBL positive isolates were found in (13 out of 28 isolates).Pure AmpC production was found in 6 of 42 isolates ().Metallo-βlactamases (MBL) production was found in only 2 of 42 isolates (). Co-production of ESBL+ AmpC was seen in 3 of 42 isolates (). All ESBL positive isolates showed multidrug resistance pattern. Detection of β-lactamase producing isolates will provide knowledge regarding resistance pattern of bacterial strains in a particular geographical area. This will further help promote appropriate and judicious use of antibiotics. | Detection of various β-lactamases and their co-production by novel disc placement method among gram negative bacilli isolates from patients with spontaneous bacterial peritonitis
