An experiment was conducted to study the nutritional requirement and effect of physiological parameters like temperature and pH on mycelial growth of different isolates of Macrophomina phaseolina causing dry root rot of chickpea. Five different solid media, five temperature range and five pH levels were tested for their effect on mycelial growth. All tested culture medium, temperatures and pH levels supported the mycelial growth of different M. phaseolina isolates. The potato dextrose agar was found better for mycelial growth of all the isolates followed by Czapek’s dox agar. M. phaseolina isolates could grow over a wide range of temperature . 200C to 400C but optimum growth was observed at 300C temperaturefollowed by 350C, 250C, 200C and minimum at 400C. The pH requirement of the M. phaseolina were showed that all the isolates could grow over a wide pH range of to but the optimum pH for its growth was found to be pH followed by pH . The growth of the fungus was reduced both below and above the optimum pH value. | Nutritional and physiological requirement of Macrophomina phaseolina (Tassi) Goid. causing dry root rot of chickpea
