MBL producing members of family Enterobacteriaceae have been responsible for numerous outbreaks of infection throughout the world. A total of 108 non repetitive carbapenem resistant strains isolated from various clinical samples, identified by standard microbiological techniques and antimicrobial susceptibility pattern was determined. MBL detection was done by combined disc test using ceftazidime-EDTA, imipenemEDTA and meropenem-EDTA. Ceftazidime-EDTA detected 90 () isolates, imipenem-EDTA detected 14 () isolates and meropenemEDTA detected 13 () isolates showing MBL production. The combined disc test using inhibitors like EDTA was found to be sensitive in detecting MBL producers, although the results vary with different antibiotic discs used. Detection of MBL among Carbapenem resistant Enterobacteriaceae in a tertiary hospital using combined disc test. | Detection of MBL among carbapenem resistant Enterobacteriaceae in a tertiary hospital using combined disc test