The aim of study was development of industrially and agriculturally important bacterial strains which produces antimicrobial protease. It is a kind of study that tends to contribute the potential Pseudomonas strains that may be helpful as plant growth promoting, biocontrol agent and may be used for the production of antimicrobial protease at industrial level. In the present study we have tried to isolate the fluorescent Pseudomonas sp. from three medicinal plants ., Achyranthus aspera, Elaegnus umbellate and Heracleum candicans. Three strains were isolated ., A4, H5 and E6, which were showed the high production of antibacterial, antifungal and proteolytic activity. The enzyme protease was purified from the strain H5 by using Sephadex G-75 column chromatography. Kinetic study of the purified protease was done by checking the effect of pH and temperature, substrate concentration, enzyme concentration and reaction time. Purified proteases can be exploited for industrial use as it is in high demands in different industries. | Purification of industrially important Proteases from Pseudomonas strain and its kinetic studies
