A field experiment was carried out during the winter season at Crop Research Farm, Department of Horticulture, SHIATS, Allahabad, (.) India. The maximum number of leaves per plant () was recorded with treatment T9 ( tones poultry manure ha-1 + GA3 150 ppm l-1 the maximum fruit weight (g / fruit), maximum fruit index (Ratio of fruit length and fruit diameter), the maximum specific gravity of fruits, the maximum total soluble solid (. 0Brix) of fruits, the maximum pH of fruit juice, the minimum acidity (%) of fruit juice, the maximum Vitamin –C content of fruits (mg / 100g) and the maximum juice content (%) of fruits was recorded in T9 : RDF 100% (150 : 75 : 100 kg ha-1 ) + Azotobacter 6 kg ha-1 + PSB 5 kg ha-1 with ( g).The minimum fruit weight (g / fruit) ( g), minimum fruit index (, minimum specific gravity of fruits(), minimum total soluble solid (. 0Brix) of fruits (), minimum pH of fruit juice (), minimum acidity (%) of fruit juice (), minimum Vitamin –C content of fruits (mg / 100g) (), minimum juice content (%) of fruits () was recorded in T0 (control). | Effect of bio fertilizers with different levels of N, P and K on physico-chemical properties of strawberry (Fragaria x ananassa Duch.) cv. Chandler