Exponential cost reduction in sequencing with advances in Next Generation Sequencing (NGS) technologies has contributed to rapid genotyping technology innovations. Genome Complexity Reduction Methods such as Restriction Associated DNA Sequencing (RAD-seq) and Genotyping-by-Sequencing (GBS) have emerged as a strong genotyping tool capable of identifying, sequencing and genotyping not hundreds but thousands of markers across almost any genome of interest, but also numbers of individuals in a single, simple experiment in a population. GBS currently uses a low coverage NGS-backed sequencing protocol for genotyping large populations and more accurately Genotype and phenotype association. Wide proportion of missing data points due to low coverage of sequencing, management and analysis of large amounts of sequence data are few potential drawbacks of GBS. But with further increase in sequencing performance, these techniques will be further improved by the availability of more reference genomes and advances in the bioinformatics field. GBS is however versatile, fast and low-cost, making it an ideal tool for many applications and addressing many plant breeding and genetics issues. The most popular application of the GBS method is linkage mapping, GWAS, marker-assisted and genomic selection, assembly of genomes and improvement and complexity of crops with different genome sizes. | Genotyping-by-Sequencing (GBS): An hi throughput genotyping approach for marker-trait association analysis
