Petrochemical is the fastest growing industry, also manufacturing variety of chemicals. However the waste generated during the manufacturing is a major environmental concern due to its persistence in soil and water bodies. An alternative to chemical treatment is bioremediation which aims to enhance the normal processes of microbial degradation and clear the pollutant from the environment. Cow dung is a nitrogen-rich material and is of economic importance as organic fertilizers and also contributes diverse species of microorganisms which further has capability to degrade pollutants like hydrocarbon. Therefore an attempt was made to evaluate potential of the bacteria isolated from cow dung for hydrocarbon degradation in MSM media. Two bacterial isolates CD-5 Bacillus sp. BBMRH (KF175230) and CD-7 Pseudomonas sp. BBMB (KF965279) were found effective and further utilised for degradation of diesel, petrol, kerosene, and petrol-ether.