Chickpea (Cicer arietinum L.) is most important pulse crop of India in term of area and production and is extensively grown from human consumption mainly in South Asia and Middle East. Chickpea (Cicer arietinum L.) is a cool season legume crop and is grown in several countries worldwide as a food source. Molecular markers and variation in genomic DNA sequences have played a major role in varietal characterization and improvement of many crop species and have contributed to our abilities to assess biodiversity, reconstruct accurate phylogenetic relationships and transferring of particular traits. The polymorphism % ranged from % (OPX-2) to 100% (OPB-6, OPX-4, OPB-12, OPA-17, OPA-11). The dendrogram was generated and 20 genotypes were aligned in 3 groups . A has 10 genotypes, B has only one genotype and C has 9 genotypes. For molecular characterization of 20 genotypes, 10 RAPD primers showing polymorphism were used. | Molecular characterization of heat tolerance chickpea (Cicer arietinum L.) genotypes using random amplified polymorphic DNA (RAPD) Markers