The present investigation was conducted to optimize an efficient callus induction protocol in Carica papaya (cv. Pusa Nanha). Two different types of explants were collected from field grown papaya plants under study viz., leaf and petiole. The explants were surface sterilized and excised into 1-2 cm diameter and inoculated on basal MS medium supplemented with various concentrations of NAA, Kinetin and TDZ. The cultures were kept in dark inside the culture room at 25 ± 2°C and humidity 60-65%. | Effect of different plant growth regulators on in-vitro callus induction in carica papaya (cv. Pusa Nanha)
