A pot culture experiment was conducted in the year 2012-2013 to test the performance of glycerol based inoculant of Bradyrhizobium on growth of soybean using variety JS-335, at Plant Pathology Department, College of Agriculture, Nagpur. Experiment was laid out in Completely Randomized Design (CRD) with eight treatments and three replications. During experiment, root and shoot length as well as number of nodules were measured at 15, 30 and 45 days after inoculation. Among all treatments, maximum root and shoot length was recorded in T2, ., 35 ml glycerol + 65 ml Bradyrhizobium broth ( cm and cm/ plant) respectively. However, maximum nodulation was recorded in T3, ., 35 ml glycerol + 65 Bradyrhizobium broth (). All the treatments showed a significant difference over untreated plot. | Effect of Bradyrhizobium broth on growth of root, shoot and nodule of soybean