The cytotoxicity and virus titration of chitosan nanoparticles encapsulated live PPR virus vaccine (PPRV-CS-NPs) was analyzed in this study. Live attenuated PPR virus vaccine (AR-87) was encapsulated in chitosan nanoparticles using ionic gelation technique. The PPRV-CS-NPs were tested for cytotoxicity study in Vero cells with different concentrations (1-40 g).The cell viability was more than 80 per cent with all the concentration of the PPRV-CS-NPs tested. This indicates that the PPRV-CS-NPs are not toxic even in concentration higher than the required vaccine dose. The viability of PPRV (AR-87) in the PPRV-CS-NPs were tested by releasing the virus from nanoparticles by trypsin digestion. The virus titration was carried out in Vero cells and the titre was found to be 10 indicating that chitosan encapsulation was able to maintain virus infectivity. | Study on cytotoxicity and virus titration of chitosan nanoparticles encapsulated live PPR virus vaccine
