This research was conducted during 2015-16 and 2016-17 kharif seasons in the Department of Horticulture, S. K. N. Agriculture University, Jobner, Jaipur (Rajasthan) to measure the influence on various physiological parameters of vegetable cowpea legume crop using growth substances and bio-fertilizers. The highest relative leaf water content (), transpiration rate ( mol H2O/m2 /s) and leaf diffusion resistance ( cm-2 ) were obtained in Brassinoids @ ppm whereas lowest leaf osmotic potential ( –bar) was recorded under Salicylic acid @ 200 ppm with significant differences in the two seasons. Among the biofertilizers, inoculation with Rhizobium dominated over PSB and No– inoculation for maximum relative leaf water content (), transpiration rate ( mol H2O/m2 /s) and leaf diffusion rate ( s cm-2 ) and minimum leaf osmotic potential ( –bar) in pooled mean of two seasons. The inoculation of PSB remained statistically at par to Rhizobium for relative leaf water content and transpiration rate. | Influence of plant growth substances and bio-fertilizers on physiological parameters of vegetable cowpea [Vigna unguiculata (L.) Walp]