In the context of the present clinical scenario urinary tract infections are to be dealt with most frequently. The non judicious haphazard use of antibiotics is to be blamed for coming into existence of resistant microorganisms. A myriad of microorganisms cause urinary tract infections. Moreover the antibiotic susceptibility pattern of the isolated uropathogens is changing continuously. Hence this study is undertaken with the objective, to study the microbial profile and to analyze the antibiotic susceptibility patterns of bacterial strains isolated from the patients with urinary tract infections (UTI). Here this study is a retrospective analysis of culture reports of urine samples. This study was undertaken at Microbiology laboratory of tertiary hospital. Age, gender, organisms isolated and their susceptibility pattern is contained in the data procured from the laboratory register. The overall prevalence of UTI in relation to both the genders was about . Among 550 urine samples collected, 250 samples revealed the significant bacterial growth, comprising 76 () samples from males and 174 () samples from females. | Bacteriological profile and antibiogram of uropathogens-a retrospective analysis