The experiment was carried both in in vitro and in situ during 2017-18 at Sam Higginbottom University of Agriculture Technology and Sciences, Allahabad to evaluate the efficacy of Trichoderma isolates against Pythium sp. Under in vitro condition the results revealed that Trichoderma sp. (isolate 5) effectively inhibited the radial mycelial growth of Pythium sp. ( %). Under in situ conditions Trichoderma isolates were tested against the damping off (Pythium sp.) disease of radish. Under in situ condition application of Trichoderma asperellum (isolate 2) showed maximum germination percentage ( %) in radish seeds. | Management of damping off (Pythium sp.) in radish by different isolates of Trichoderma spp.