The present study was envisaged to assess the effect of different seasons on semen quality of crossbred bull semen. Frozen semen samples produced from Kerala Livestock Development Board was collected from three different seasons of Kerala. Semen collected from six crossbred bulls of same exotic inheritance was selected. Maximum temperature and relative humidity significantly varied between different seasons (p≤). However, Temperature Humidity Index (THI) and minimum temperature did not vary between seasons. After thawing, semen from each season was subjected to different in vitro tests like motility, viability, abnormality and acrosome integrity. Semen cryopreserved during summer season had significantly lower motility, viability, acrosome integrity (p≤) and higher abnormality than rainy and post monsoon season. It can be concluded that semen cryopreserved during rainy season is having superior quality than other two seasons and post monsoon is intermediate between rainy and summer. | Relationship between season and semen quality in crossbred bull semen