Damping-off is a serious disease condition caused by fungi affecting seeds and seedlings in both nursery and field conditions. An around 40 to 50, damping-off affected diseased sample of onion seedlings were collected from the onion crop nursery and the adjoining main fields in and around Udaipur district of Rajasthan. These collected samples were subjected for the fungal isolation, on potato dextrose agar (PDA) medium under the invitro condition. Fungi with the distinct colony were picked up and subjected for pure culturing. The isolated fungi were identified as Fusarium solani, Sclerotium rolfsii. | Isolation and identification of fungi causing damping-off disease in onion (Alium cepa L.) plants