Pleurotus sajor-caju, a nutrient rich mushroom was cultivated on four different substrates, viz. Wheat straw, Paddy straw Maize stalk and Typha Leaves substrates. It was observed Wheat straw followed by paddy straw Wheat straw, Maize stalk and Typha Leaves. The third harvesting was complete in 42 days in case of wheat straw, which was statistically more compared to paddy straw and maize stalk. The effect of various substrates on the yield of fresh mushroom the result indicated that higher yield of g ½ kg was obtained on wheat straw followed by paddy straw and maize stalk, the minimum yield of basidiocarp of 285 g ½ kg was recorded in Typha Leaves. | Harvesting period and yield of pleurotus sajor-caju on different agro-substrate