Otitis Media is inflammation of the middle ear, which often causes hearing loss. It is one of the common ear infections addressed very frequently. Appropriate treatment at right time is necessary to prevent adverse complications. The common bacteria causing Otitis Media are Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter species, Enterobacter species, Escherichia coli, Proteus mirabilis and common fungi causing otitis media are mainly Candida non albicans and Aspergillus species. The aim of the study was to isolate the common bacterial and fungal agents causing Otitis Media and to find the antibiotic susceptibility pattern of those bacterial isolates. A retrospective study was carried out in a tertiary care hospital on patients with otitis media. Samples received in the lab and processed by standard methods were analyzed. A total of 79 samples were analyzed during the period of 1 year from Jan 2018 to Dec 2018. On statistical analysis, male patients were more prone to develop infection than the female patients. Also patients of the age group 51 years and more were affected mainly. Among the bacterial isolates, Pseudomonas aeruginosa were found to be predominant followed by Staphylococcus aureus. Among the fungal isolates, Candida non albicans were found to be predominant, followed by Aspergillus species. Majority of the bacterial gram positive isolates were found to be sensitive to Vancomycin, Linezolid and gram negative isolates were found sensitive to combination drugs Piperacillin-tazobactum. With time the bacteria are developing resistance to the antibiotics. Hence treatment at the appropriate stage with suitable drug and dose is significant in preventing complications due to Otitis Media.