The aim of present study was to isolate, identify various species of Candida responsible for producing clinical Candidacies from blood culture and to determine antifungal susceptibility pattern of Candida species by disc diffusion method. study was conducted at Microbiology department Medical college Rajkot from March 2014 to June 2015. Blood samples from suspected cases were inoculated in to Brain Heart Infusion broth were incubated at 370C and subcultured routinely. Whenever growth of white opaque colony on Blood agar was noticed, further processed to identify isolated yeast species by Gram’s stain, germ-tube production, Hichrome and cornmeal agar morphology. | Characterization of isolated candida spp in blood stream infection and its antifungal susceptibility pattern