Present study on NDV was carried out at Department of Veterinary Microbiology, LUVAS, Hisar. As we all know that Newcastle disease is also known as Ranikhet disease in India. Virus causes a worldwide disease of birds . chickens, turkeys, guinea fowl, pheasants and pigeons. Man is susceptible and suffers from self limiting conjunctivitis. It is transmitted by droplet, direct contact, fomites or the ingestion of excreted virus and its average incubation period is 5-6 days but it may vary from 2-15 days. In severe outbreaks the symptoms appear within 3 days. So efforts had been made to culture, detect and confirm NDV virus from 15 tissue samples. Only two out of 15 samples and La Sota vaccine strain could be confirmed to contain NDV on the basis of HA and HI tests. | Culture and detection of NDV virus by haemagglutination test (HA) and haemagglutination inhibition test (HI)
