The aim of this study was to evaluate the efficacy of whey protein isolate (WPI) as an encapsulation matrix for improvement of L. fermentum 39-183 viability to low pH and bile and releasing the encapsulated bacteria in ex vivo porcine gastrointestinal (GI) contents. 1g of protein microcapsules (≈ 108 CFU of L. fermentum 39-183 or E. coli GFP+ ) were incubated in ex vivo porcine GI contents (9 mL) under anaerobic conditions at 37oC. | Use of whey protein for encapsulation and controlled release of probiotic bacteria from protein microencapsulate in ex vivo porcine gastrointestinal contents