In this study, phytochemical investigation of the n-hexane and ethyl acetate extract of leaves and twigs of Urena lobata L. led to the isolation of β-sitosterol (1), β-sitosterol3-O-β-D-glucopyranoside (2), 2-acetylamino-3-phenylpropyl 2-benzoylamino-3-phenylpropanoate (3), quercetin (4), and trans-tiliroside (5). Their chemical structures were determined by spectroscopic methods, including MS, 1D-, 2D- NMR, and by comparing their spectral data with those reported in previous papers. Compounds 3 and 5 were isolated for the first time from Urena lobata L. | Study on the chemical composition of urena lobata growing in Viet Nam