The phenotypic changes in target cell induced by an exogenous agent, . biologically active chemical, can be detected by labelling selected organelles with fluorescent dyes and observed under the high-throughput microscope. The changes of known biological targets (. nucleus, proteins, mitochondria, microtubules, etc.) induced by a compound of interest allow predicting its mechanisms of action. High-throughput microscopy with capability of multiparametric imaging in combination with automated image-analysis is referred to as highcontent analysis or high-content screening (HCS). | Application of image-based high content analysis for the screening of bioactive natural-products
