Groundnut (Arachis hypogaea L.) production is constrained by a myriad of biotic and abiotic stresses which necessitate the development and use of superior varieties for increased yield. Germplasm characterisation both at the phenotypic and molecular level becomes important in all plant breeding programs. The aim of this study was to characterise groundnut genotypes at molecular level using simple sequence repeats (SSR). A total of 30 SSR markers were screened and 20 were found to be polymorphic with an average polymorphic information content (PIC) value of . Of the 66 groundnut genotypes studied, 57% showed very close relationship (~80% similarity) with one or more genotypes among themselves. | Assessing the molecular diversity in groundnut (Arachis hypogaea L.) genotypes using microsatellite-based markers