Treatment of postpartum lactating anestrous Ongole cows (n=8) using G6G hormonal protocol was undertaken. Following the treatment, 7 cows exhibited estrus after second PGF2α injection. The estrus detection rate was (7/8) per cent. Mean time to onset of estrus was observed as ± h. The per cent of cows that showed intense, intermediate and weak estrus were , and per cent. The peak estrus synchrony with highest number of animals exhibiting estrus between 24 to 48 h was (4/7) per cent. The artificial insemination submission rate was (7/8) per cent. | Evaluation of G6G synchronization protocol in the treatment of postpartum anestrous ongole cows