Layer chicken, Marek’s disease, PCR, Histopathology Eight dead commercial layer birds of 38 weeks age were examined to know the cause of death. The total flock size was 10,000 and birds were raised in cages from day one of age. The total mortality over a period of 13 weeks was per cent. The birds appeared weak and emaciated. At necropsy, liver, spleen, kidney revealed tumour nodules. The tumours were firm in consistency and smooth when cut. Histopathology of liver, spleen and kidney were corroborated the gross lesions. Marek’s disease (MD) was confirmed by conducting polymerase chain reaction (PCR).
