Lactic acid bacteria are described as heterogeneous group of regular, gram-positive, rod shaped, non-motile, non-spore forming bacteria with absence of catalase enzyme. Laboratory study was undertaken to isolate and characterize the lactic acid bacteria from silkworm gut in phenotype basis. A total of 21 silkworm gut samples were collected from silkworm breeds, Double hybrid {(CSR 6 X CSR 26) X (CSR 2 X CSR 27)} obtained from Chawki rearing center (CRC) at Annur, Coimbatore. The bacteria samples were isolated after growth on MRS agar medium and pure culture was obtained by sub-culturing on the same medium. | Isolation and characterization of indigenous probiotic bacteria from silkworm (Bombyx mori L.) gut