Corynebacterium sp. is a Gram-positive, non-motile and facultatively anaerobic bacilli present in the environment as ubiquitous organism. A 45 day-old Holstein Friesian calf from University Livestock Farm (ULF), Kerala Veterinary and Animal Sciences University, Mannuthy was reported with joint ill in both of its forelimbs. Using aseptic procedures, synovial fluid was collected from the right knee joint and inoculated on to blood agar and incubated at 37⁰ C for 24 to 48 hrs to isolate and identify the bacterial aetiology involved. Pin-point gray colonies with hazy haemolysis could be observed. On Gram’s staining, Gram-positive bacilli with palisade arrangement could be observed. On sub culturing on to Potassium tellurite blood agar, black coloured colonies suggestive of Corynebacterium sp. was observed. | Isolation and identification of corynebacterium jeikeium from synovial fluid of a joint Ill affected calf