A survey had been conducted in districts of South 24 parganas, Nadia, Barddhaman and Jalpaiguri of West Bengal, India on shoot fly infestation of bamboo. Acroceratitis parastriata David & Hancock, Rioxoptilona dunlopi (Wulp) (Diptera: Tephritidae) and Neosilba sp. (Diptera: Lonchaeidae) a saprophytic species could be recorded on young shoots of bamboo. Amongst the infesting species, Acroceratitis parastriata David & Hancock had been found to be predominant. Infestation of the pests caused severe damage of newly emerged shoots of the crop, resulting in oozing, stunted growth with hollow internode and drying up of the entire shoots. The activity of the flies could be recorded during day. Maximum pest attack took place when bamboo shoots were of 15-20 cm height. | Occurrence of dipteran shoot flies, on bamboo in West Bengal, India