The nematicidal effect of Purpureocillium lilacium, Pochonia chlamydosporia, Pseudomonas fluorescens singly or in combination were tested against root-knot nematodes under open field conditions. In the present study, the efficacy of four biocontrol agents was tested against Meloidogyne spp. The results revealed that soil application of vermicompost @ tons/ha enriched with P. lilacinum @ kg + vermicompost @ tons of /ha enriched with P. fluorescens @ kg/ha (T4) proved effective to manage root-knot nematode, Meloidogyne spp. and hence increased bitter gourd fruit yield as compared to other treatments.