Considerable losses caused by Alternaria blight on the oilseed crop rapeseed-mustard annually caused by Alternaria brassicae and Alternaria brassicicola. The study was conducted with the aim to assess tolerant sources of mustard lines against dark spot and to ascertain the optimum time of screening against the disease in Bihar region. We screened 50 lines of mustard against Alternaria blight under natural incidence in the year 2015-16 and 2016-17 using Area Under Disease Progress Curve (AUDPC). | Screening of mustard lines against alternaria blight and optimization of screening time under cool humid conditions of Bihar, India