Bovine viral diarrhoea virus (BVDV) is the most prevalent infectious disease of cattle. The envelope glycoprotein (E2) gene of an Indian BVDV isolate was already cloned. In this study, recombinant plasmid was used to transfect 70-80% HeLa cell, expressed E2 protein which was confirmed by immunoperoxidase test. Rabit anti mouse HRPO conjugate was used in expression analysis and anti BVDV-E2 hyperimmune serum was raised in mice using recombinant plasmid for develop specific antibody. | Expression of bovine viral diarrhoea virus E2 gene in hela cell line
