Riemerella anatipestifer, a pathogen causing infectious serositis and septicaemia in ducks, turkey, geese, pheasants, swans, poultry and also reported in pigs, which belongs to family Flavobacteriaceae. Twenty one serotypes have been reported so far by serum agglutination test which is found to be labour intensive and time consuming. In order to find out the better method to differentiate the isolates, PCR-RFLP and REP-PCR has been compared. The 20 isolates of R. anatipestifer were subjected to 16S rRNA PCR and subjected to restriction digestion with Hae III and the banding pattern was observed for differentiation. | Comparison of polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) and repetitive sequence-PCR (REP-PCR) for differentiation of Riemerella anatipestifer isolates
