Citrus, one of the most important group of fruit crops around the world, are propagated at large scale with many difficulties. Propagation through seeds is challenging because of Phytophthora foot rot together with recalcitrance of citrus seeds. Vegetative propagation of Citrus species is mainly performed now-a-days by budding on seedling rootstocks. As heavy losses are experienced among the susceptible seedlings due to Phytophthora and Citrus tristeza virus (CTV), the interest in resistant rootstocks has greatly increased. The potential of conventional methods of citrus plant breeding of rootstocks are limited by physiological factors such as heterozygosity, inbreeding depression, nucellar polyembryony and juvenility. Under such conditions advanced tissue culture techniques provide best possible alternative for producing large number of resistant progenies from elite citrus genotypes. Plant tissue culture provides reliable and economical method of maintaining pathogen free plants that allows rapid multiplication and international exchange of germplasm. | In vitro propagation of citrus species through callus induction and regeneration: A review
