Cow dung has a wide range of micro-organisms, like- endospore forming Bacillus, Enterococcus & protozoa. In the present investigation attempts were made to isolate bacteria using nutrient agar media (NAM). The isolated bacteria were identified on the basis of their colony characteristics, morphology, Gram’s staining and biochemical test. It was observed that spore forming bacillus were the predominant type of organism, which possibly helped in maturation of manure forming.