The main concern in RNA research is the risk of degradation of RNA in the experimental conditions due to the ubiquitous nucleases and detecting the RNA quality through RNA integrity score provides better understanding of degradation process. Conventionally, the total RNA quantity and purity measured using spectrophotometer but even a standard reading stands questionable due to contributions from protein and genomic DNA and doesn’t ensure appropriate discrimination among RNA fractions. | Chicken RNA integrity assessment through capillary electrophoresis for miRNA expression study using RT-qPCR
