Carbon and nitrogen sources were selected to create a new modified Chapek-Doks nutrient medium in order to increase the production of secondary metabolites, that inhibit pancreatic α-amylase, by endophytic fungi Penicilliumbrevicaule alba-CC200 and Aspergillus egypticus-HT166. The results showed that the highest α-amylase inhibitory activity - and 88%, respectively, was observed during the shake flask cultivation of strains at the end of the stationary phase on a medium containing casein supplemented by yeast extract. | Effects of fermentation conditions on the production of secondary metabolites of penicillium brevicaule alba-CC200 and aspergillus egypticus–HT166 inhibiting pancreatic α-amylase