Mastitis is the most economically significant disease of dairy animals. This condition is associated with a significant reduction in milk yield, increased costs of production and deteriorated milk quality. In the present study a total number of 40 bovine mastitis milk samples were collected to isolate and identify the Pseudomonas aeruginosa. All the samples were inoculated into nutrient broth which was further cultured on agar plates. Colonies showing typical Pseudomonas morphological characteristics were processed for biochemical analysis. | PCR based detection of pseudomonas aeruginosa in mastitic cow milk