The objective of the current experiment was to investigate the various semen characteristics of two indigenous breeds of chicken namely Nicobari and Ankleshwar, as such data were not yet reported. Freshly ejaculated semen samples were collected from twenty breeder roosters of each breed and subjected to physical and biochemical analysis. Immediately after collection, the semen volume of individual birds was recorded using tuberculin syringe and further analysed for seminal motility, concentration and percentage of live, dead and abnormal spermatozoa using standard procedures. | Influence of indigenous nicobari and ankleshwar chicken breeds on physical and biochemical attributes of semen