Nghiên cứu tác dụng ức chế enzym α-glucosidase và quét gốc tự do DPPH của cao chiết hạt cà phê xanh (Coffea canephora)

Nghiên cứu này đánh giá tác dụng ức chế enzym α-glucosidase và tác dụng chống oxi hóa thông qua khả năng quét gốc tự do DPPH của cao chiết hạt cà phê xanh (Coffea canephora) và các phân đoạn. Hạt cà phê được nghiền nhỏ, chiết siêu âm bằng ethanol 70% và tiến hành phân đoạn lần lượt với n-hexane, ethyl acetate (EtOAc) và n-butanol (n-BuOH). | Nghiên cứu tác dụng ức chế enzym α-glucosidase và quét gốc tự do DPPH của cao chiết hạt cà phê xanh (Coffea canephora) VNU Journal of Science: Medical and Pharmaceutical Sciences, Vol. 35, No. 2 (2019) 12-18 Original Article Study on α-glucosidase enzyme inhibitory activity and DPPH free radical scavenging of green coffee bean extract (Coffea canephora) Dang Kim Thu1, Vu Manh Hung2, Nguyen Thi Trang1, Bui Thanh Tung1,* 1 VNU School of Medicine and Pharmacy, 144 Xuan Thuy, Cau Giay, Hanoi, Vietnam 2 Buon Ma Thuot University, 298, Ha Huy Tap, Tan An District, Buon Ma Thuot City, Dak Lak Received 07 October 2019 Revised 10 October 2019; Accepted 16 October 2019 Abstract: α-glucosidase enzyme is one of the important molecular targets in the treatment of diabetes. In addition, free radicals are the cause of insulin resistance, damage β- cell pancreatic, glucose uptake disorders and induced diabetes. In this study we evaluated the inhibitory effect of α- glucosidase enzyme and antioxidant effect by using DPPH free radical scavenging method of green coffee bean extract (Coffea canephora) and its fractions. Coffee beans were pulverized, extracted with ethanol 70% by sonications, and fractionated with n-hexane, ethyl acetate (EtOAc) and n- butanol (n-BuOH) solvents. Our results showed that coffee bean extract has a strong α-glucosidase enzyme inhibitory activity, especially EtOAc fraction with an IC50 value of ± µg/mL. Furthermore, the coffee bean extract has an antioxidant effect by DPPH radical scavenging ability, and EtOAc fraction has the highest effect with an IC50 value of ± µg/ml. Our results show that green coffee beans and EtOAc fraction have potential effect in preventing and supporting for the treatment of diabetes. Keywords: Coffee; Coffea canephora; free radical; α-glucosidase; DPPH. _ Corresponding author. Email address: tungasia82@ .