Serum gonadotropin @50 and 100IU/ml, estrodiol-17β @ and 1μg/ml, EBS @5 and 10% and BFF @5 and 10% supplementation Iis done on cleavage rate of buffalo oocytes for the comparison of efficacy of hormones. Ovaries were collected from buffaloes slaughtered at local abattoir. Oocytes were aspirated and good and excellent quality oocytes were matured in basic maturation media TCM199 supplementing above mentioned supplements separately. | Comparison of cleavage rate of buffalo oocytes on supplementing with different concentration of serum gonadotropin, estradiol 17b, estrus buffalo serum and buffalo follicular fluid
