A cow has high socio-cultural values, plays significant role in rural economy, represent cattle wealth and bio-diversity. Cow urine is the most effective substance of animal origin with innumerable therapeutic values. Various kinds of pathogens such as bacteria, fungi, viruses, nematodes, and mycoplasma causing diseases remain a major threat to public health, despite tremendous progress in human medicine. Many researches have been done and have shown cow urine use for treatment of skin diseases, stomach diseases, diabetes, etc.