Hair follicle is an ideal system for studying the biology and regulation of adult stem cells. Multipotent stemness of the canine hair follicle stem cells (cHFSCs) were detected by immunostaining the cells with positive HFSC surface markers like Anti-cytokeratin-15 (CK15) and CD200. In this study, canine hair follicular cells were cultured in Williams’E media supplemented with growth factors. | Fluorescence activated cell sorting (FACS) of CK15 cells in canine hair follicular tissue