The molecular marker technology has a great potential for assessing genetic diversity and relationship among the selected varieties. In the present study 52 germplasms of green gram showing distinct morphological differences were screened using 15 ISSR markers. The DNA was extracted from the green leaf samples collected. Modification in extraction procedure resulted into better and clear banding pattern when subjected to PCR analysis. | Assessment of genetic diversity and to study the relationship in selected green gram germplasm by ISSR Marker