Ocimum sanctum L. or “Tulsi” or Holy basil is a plant well known for its medicinal properties. This plant is rich in essential oil sequestered in peltate glands. The Tulsi is rich source of eugenol, methyl eugenol, chavicol, ursolic acid, rosmerinic acid and other phenolics and terpenes. Aloe barbadensis and Plantago ovata are plants rich in polysaccharides. These plants are very important medicinal plants. A. barbadensis is source of aloin which is used in various cosmetic products and P. ovata is the main source of Isabgol-Husk. | Development of liquid nitrogen free and efficient DNA isolation method for plants rich in volatile oil, secondary metabolites and polysaccharides