A study was carried out to assess the impact of shelter designs on the production performance of dairy cattle in the three selected agro – climatic zones of Tamil Nadu. viz., Southern, Cauvery delta and High rainfall zones. The effect of various shelter designs on the activity of liver enzymes and stress hormones were analyzed. The serum samples were collected throughout the year . in all the four seasons, viz., winter, summer, south-west monsoon and north-east monsoon. | Impact of different shelter designs on selected serum enzyme activities and on concentration of stress hormones in dairy cattle of Tamil Nadu, India