Mycorrhiza is important fungi for agriculture. To study of that plant’s root and soil samples were collected from the different regions of junagadh and near to junagadh district. AMF spores were isolated by Wet-sieving-decanting method and AdhesionFlotation Technique. Morphological studies followed by tryphan blue staining revealed different stages with different components of AM fungi in compound microscope. | Morphological and molecular identification of Arbuscular mycorrhizal (AM) fungi