Analysis of the genetic stability of banana tissue culture propagated plantlets cv. ney poovan (AB) using morphological and molecular markers

The present study was carried out to monitor somaclonal variations of banana cv. Ney Poovan (AB) during micropropagation using random amplified polymorphic DNA (RAPD). Sword suckers were used as explants and regenerated on MS media supplemented with BAP alone, BAP + Kinetin and TDZ at varying levels of concentrations. The regenerated shoots were again subcultured on MS medium fortified with plant growth regulators at different concentrations. Rooting was obtained on ½ MS medium supplemented with mg/l IBA and 2 g/l of activated charcoal. Rooted plantlets were observed visually for phenotypic appearances and then transferred in greenhouse for hardening. After one month the hardened plants of banana were screened for genetic stability using RAPD primers. | Analysis of the genetic stability of banana tissue culture propagated plantlets cv. ney poovan (AB) using morphological and molecular markers

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