Central venous catheter insertion is a necessity while dealing with patients in MICU due to associated comorbid conditions. But following sterile barrier precautions while inserting and handling central line catheters is much more important as infectious complications take an upper hand if these “central line bundles” are not followed with great care. A prospective study was carried in a tertiary care hospital. The central line bundles while inserting the catheter with site and type was studied. Fifty (50) patients developed Catheter related local infection (CRLI) and seven (6) patients developed Central line associated blood stream infections (CLABSI). | Universal precautions in preventing central venous catheter associated blood stream infections. a study at tertiary care hospital of ‘Central Line Bundles’ in MICU