The experiment was carried out on gladiolus variety ‘Psittacinus Hybrid’ to know the best mutagen between physical mutagen (Gamma rays) and chemical mutagen (DES). The experiment was carried out at Floriculture Research Farm, ASPEE college of Horticulture and Forestry, Navsari during the year 2013-2014. | Comparative effect of physical and chemical mutagens in inducing variability in Gladiolus variety ‘psittacinus hybrid’